Because it was a mom who tested this stuff in her kitchen yes?
>The testing of drinking water, urine and breast milk was carried out by Microbe Inotech Laboratories, Inc. (MiL inc.)
>For the detection and quantitation of glyphosate in water (groundwater, surface water, well water), urine and breast milk, the MiL inc. uses a 96 well microtiter plate assay. For soil, crop, and foods, additional preparation steps are required but can be processed at a small additional fee. This assay applies the principles of enzyme linked immunosorbent assay methodology (ELISA) to the determination of glyphosate.
>The sample to be tested is derivatized and then added, along with an antibody (binding protein)specific for glyphosate to microtiter wells coated with Goat Anti-Rabbit Antibody and incubated for 30 minutes. A glyphosate enzyme conjugate is then added. This particular format is known as a competitive ELISA assay since, at this point in the procedure, a competitive reaction occurs between the glyphosate which may be in the sample and the enzyme labeled glyphosate analog for the antibody binding sites on the microtiter well.
>The reaction is allowed to continue for sixty minutes. After a washing step and addition of a substrate (color solution),a color signal (blue color) is generated. The presence of glyphosate is detected by adding the “Color Solution”, which contains the enzyme substrate (hydrogen peroxide) and the chromogen (3,3’,5,5’- tetramethylbenzidine). The enzyme -labeled glyphosate bound to the glyphosate antibody catalyzes the conversion of the substrate/chromogen mixture to a colored product.
>After an incubation period, the reaction is stopped and stabilized by the addition of a diluted acid (Stopping Solution). Since the labeled glyphosate (conjugate) was in competition with the unlabelled Glyphosate Testing Method: Glyphosate Plate Assay 15 glyphosate (sample) for the antibody sites, the color developed is inversely proportional to the concentration of glyphosate in the sample.
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